Hif2a western blot
Web7 de jun. de 2024 · j Western blot analysis of OXPHOS complexes, PKA Cα, and UCP1 in iWAT from WT and HIF2α AKO mice upon re-warming (cold 2 weeks + TN 1 week) with con or miR-3085-3p mimic injection. Web27 de mar. de 2012 · RT-PCR and Western blot analysis indicated that hypoxia could up-regulate the expression of HIF-1α on both mRNA and protein levels. Hypoxic …
Hif2a western blot
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Web2 de ago. de 2010 · Littermate controls were used for all experiments conducted. Southern blot analysis of DNA obtained from BMDMs showed approximately 90% Hif2a excision in the genome of mutant macrophages (Figure (Figure1B), 1 B), consistent with previous reports for this LysM-Cre allele . Western blot analysis confirmed HIF-2α protein loss in … WebWestern blot was performed using anti-HIF2A-Polyclonal Antibody (Product # PA1-16510) a 120 kDa band was observed corresponding to HIF2A was observed across the cell …
Web18 de jun. de 2024 · (B) Western blot analysis and densitometric quantification of HIF2A protein levels in human PAECs transfected with siCTRL and siPFKFB3 under normoxic … WebDetection of Human, Mouse, and Rat HIF‑2 alpha /EPAS1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, bEnd.3 mouse endothelioma cell line, and PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 µM CoCl 2 for 16 hours. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse …
Web23 de mar. de 2024 · Therefore, we bred Hif2a deficient mice and subjected them to renal trauma through UUO surgery, ... The procedures for western blot analysis were …
WebWestern Blot tips. HIF-1 alpha can be a challenging target to work with in western blot. HIF-1 alpha is only stabilized at O2 concentrations below 5%. Under normoxic conditions, HIF-1 alpha has a short half-life and may be degraded within 5-8 minutes in both nuclear and cytoplasmic compartments. Therefore, proper sample preparation is critical ...
WebMorin A et al. TET-Mediated Hypermethylation Primes SDH-Deficient Cells for HIF2a-Driven Mesenchymal Transition. Cell Rep 30:4551-4566.e7 (2024 ... however under hypoxia HIF2 alpha is induced and the signal for HIF2 alpha in Western blot is significantly stronger compared to normoxic conditions. If you need any further assistance in ... dwht97529WebMouse HIF-2 alpha Antibody Pack. Tested Applications: Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, … dwht81424 user manualWebHIF-1 alpha Antibody (H1alpha67) [NB100-105]: Western blot (WB) analysis of lysates from Caki-1 cells which were left un-treated or were treated with 100-300 uM of CoCl 2 for 4-6 hours. The antibody detected … dwht75021 staplesWebDoes anyone have any advice on how to successfully extract HIF1a and HIF2a from HEK293T for measurement in a western blot. These are the materials I am using: … crystal hudson deathWeb15 de mar. de 2024 · Western blot analysis. MCF-7 and MDA-MB-231 were treated with selected concentration of CoCl 2 (50 µM, 100 µM, 150 µM for MCF-7 and 10 µM, 25 µM, 50 µM and 100 µM for MDA-MB-231) and … dwht77100-xj instructionsWebAfter western blot we use ECL method for detection. if you have any further queries you can ask to me anytime. Cite. 26th Aug, 2016. Muhammad Zahoor. University of Oslo. … dwht81424 manualWeb4 de abr. de 2011 · Retinal morphology and vasculature were analyzed in sections and flat mount preparations. Gene and protein expression were determined by real-time PCR and Western blot analysis. Results: The Cre-mediated knockdown caused a significant reduction in Hif1a gene expression and HIF1A protein levels in the early postnatal retina. crystal hubbs