WebDyLight™ 405 - conjugated secondary antibodies are excited maximally at about 400 nm and fluoresce with a peak at about 421 nm. They are very bright and photostable, but their optimal use requires instruments … WebThe DyLight® fluorophore spectra are shown below. Table: Spectral properties of DyLight® fluorochromes. In order to expand the spectra, please click on the spectrum …
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WebSpectrum [DyLight 405] DyLight 405 is a fluorescent compound with an excitation peak at 399 nm and an emission peak at 434 nm. It can be excited using a 405 nm laser paired with a 450/50 nm bandpass filter, a configuration that can be found, for example, in the BD Special Order LSRFortessa™ Cell Analyzer. DyLight 405 is spectrally similar to ... WebFAQs for Lightning-Link (R) Rapid DyLight 550 Kit (323-0005). (Showing 1 - 8 of 8 FAQ). Can I label a peptide/protein/antibody other than IgG? Yes. The Lightning-Link® technology works by targeting free amine groups on your target, meaning it can be used to label most biomolecules.As the protocols provided were optimized for labeling IgGs, we would … fit 2 perform warminster
Donkey Anti-Rabbit IgG H&L (DyLight® 550) preadsorbed (ab96920) - Abcam
WebForm / Storage: Conjugate supplied as a liquid in 0.1M sodium phosphate, 0.15M NaCl (pH7.4), 1% BSA, 0.02% NaN3. Upon receipt, store at 2-8°C in the dark. WebProtein A DyLight™680 has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight ... WebMar 6, 2024 · IHC-P. 1/50 - 1/500. DyLight ® 550 replaces DyLight ® 549. The DyLight ® 550 excitation is 562nm and the emission is 576nm. DyLight ® 550 provides the same orange-to-red fluorescence and photostability as the DyLight ® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight ® 549 or DyLight ® 550. can eyeballs freeze